FATT-CTL assay for detection of antigen-specific cell-mediated cytotoxicity

Preparation of prostate specific antigen standards for immunoradiometric assay

Background: Immunoradiometric assay is one of the most common and precise methods for determination of prostate specific antigen (PSA) in clinical laboratories. Usual use of human serum in routine assays has many disadvantages such as easy contamination and precipitation, instability and unavailability. Thus in order to avoid these problems the artificial matrix was used which acts sim...

PI3Kδ is indispensable for CTL-mediated cytotoxicity

Background The expression of catalytic phosphoinositol-3-kinase isoform δ (PI3Kδ) is restricted to the haematopoetic compartment. Accordingly, PI3Kδ serves as a drug target to eliminate leukaemic cells. However, we previously showed that PI3Kδ is indispensable for the function of natural killer (NK)-cells [1]. Thus, the therapeutic success of PI3Kδ inhibitors is likely to be compromised by unin...

Evaluation of antigen specific recognition and cell mediated cytotoxicity by a modified lysispot assay in a rat colon carcinoma model

BACKGROUND Antigen-specific CD8+ cytotoxic T lymphocytes represent potent effector cells of the adaptive immune response against viruses as well as tumours. Therefore assays capable at exploring the generation and function of cytotoxic T lymphocytes represent an important objective for both clinical and experimental settings. METHODS Here we show a simple and reproducible assay for the evalua...

Activation-Induced Cell Death Antigen-Specific CTL from Tumor-Mediated Human CD5 Protects Circulating Tumor

preparation of prostate specific antigen standards for immunoradiometric assay

background: immunoradiometric assay is one of the most common and precise methods for determination of prostate specific antigen (psa) in clinical laboratories. usual use of human serum in routine assays has many disadvantages such as easy contamination and precipitation, instability and unavailability. thus in order to avoid these problems the artificial matrix was used which acts similar to h...